THE UTILIZATION OF DIOSGENIN FOR MANAGING THE GENETICALLY TRANSMITTED OPTIC NERVE SHEATH MENINGIOMAS (ONSMS)

Abstract

Diosgenin is known as a steroidal saponin which was found to have effective oncological properties. However, in the past research, insufficient number foci have been given on diosgenin’s anti-proliferative effects within the context of “optic nerve sheath meningioma (ONSM) cells.” ONSMs are often asymptomatic and can be transmitted genetically. Therefore, the present research focuses on the hereditarily transferred ONSM, focusing on the role of diosgenin in influencing cell migration and invasion, autophagy, cell death (apoptosis), and progression of cell cycle in this regard. For this purpose, the WTS-1 assay was utilized for evaluating HBL-52 ONSM cells viability, while western blot and electron microscopy were used for detecting autophagy. Moreover, cell invasion and migration of the associated cells were also evaluated, using transwell as well as wound healing assays. Finally, fluorescence microscopy, flow cytometry and western blot were used for determining the apoptotic cell death. The results obtained have shown that HBL-52 ONSM viability is decreased by diosgenin due to autophagy activation. However, Beclin 1 and LC3-II expressions were also upregulated by autophagy and diosgenin resulted in arrest of cell cycle at G1 sub-phase. Moreover, the cell invasion as well as migration of HBL-52 ONSM cells were decreased by diosgenin, triggering the apoptosis which depended on mitochondria. In conclusion, diosgenin is found to have effective anti-tumoral characteristics within the ONSM cell within the in vitro situations. Thus, the present study has also been effective in providing important implications to promote the utilization of diosgenin for managing the genetically transmitted ONSM.

Keywords: Optic Nerve Sheath Meningioma; Genetically transmitted ONSM; ONSM; Diosgenin; HBL-52 ONSM; The treatment of ONSM.